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Epigenomics
Profile DNA methylation with methods suited to your study — direct native detection on long reads or base-resolution sequencing on short-read platforms.
Service options
Not sure which option matches your project? A complimentary technical consultation helps you pick based on sample quality, input and objectives.
5mC and other modifications read directly from native DNA — no bisulfite conversion, no extra prep — alongside long-read sequence, structure and phasing.
Request this serviceBase-resolution methylomes via WGBS or enzymatic conversion — genome-wide CpG coverage for cohort studies and differential methylation.
Request this serviceThe Gateway advantage
Direct ONT methylation calls on unmodified DNA, or gold-standard bisulfite/enzymatic workflows on Illumina.
Population-scale methylome studies through single-molecule phasing of methylation over structural variants.
Validated chemistries and conversion controls keep false-positive rates low.
Fluorometric quantification and fragment sizing flag compromised DNA before sequencing.
Preparation, instrument cycles and pipelines run inside verified partner facilities.
Per-CpG matrices, differentially methylated regions and browser tracks ready to publish.
Laboratory workflow & QA
Step 1
ONT native calls vs Illumina conversion chemistry matched to coverage and resolution needs.
Step 2
Ship snap-frozen tissue or cells on dry ice; purified gDNA ships at 4 °C.
Step 3
HMW DNA extraction for ONT or standard extraction for WGBS with documented QC.
Step 4
Native long-read runs or converted short-read libraries sequenced to target depth.
Step 5
Methylation calls, DMRs and tracks delivered as interpreted reports.
Beyond the FASTQ
From raw reads to regulatory insight — QC, methylation calling, differential analysis and publication-ready visualizations.
Deliverables include
FASTQ files
Sequencer QC report
Per-CpG methylation calls
Methylation tracks (bedGraph/bigWig)
Differentially methylated regions
FASTQ files
Sequencer QC report
Per-CpG methylation calls
Methylation tracks (bedGraph/bigWig)
Differentially methylated regions
Hierarchical clustering & PCA
Heatmap correlation
Metagene profiles
Publication-ready figures
Hierarchical clustering & PCA
Heatmap correlation
Metagene profiles
Publication-ready figures
Figures & files delivered with every project — hover to pause
Applications
Track methylation reprogramming across differentiation and developmental stages.
Stress memory, transgenerational methylation and trait regulation in crops.
Aberrant methylation signatures in developmental and metabolic disorders.
m6A/m4C and methylation-driven phase variation in bacterial genomes.
Methylation drift linked to exposure, diet and environmental change.
FAQs
Explore related sequencing solutions
Contact the team for a technical consultation — method selection, sample requirements and a sequencing strategy tailored to your goals.
Share your samples and goals — a doctoral-level scientist responds within one business day with a study plan and competing blind quotes.
Administered by Syntax of Life Private Limited
Request a quote