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Sanger
Sequence individual DNA fragments for confirmation and identification — the gold-standard read for validating clones, amplicons and barcodes.
Service options
Not sure which option matches your project? A complimentary technical consultation helps you pick based on sample quality, input and objectives.
Direct reads of purified amplicons — confirm sequence identity, genotype variants and edit sites with ~500–900 bp high-quality traces.
Request this serviceVerify inserts, junctions and full constructs in plasmids and engineered clones — reads across cloning sites with custom primers.
Request this serviceSpecies identification with COI, 16S, ITS, rbcL and matK markers — rapid taxonomic confirmation for biodiversity and food-authenticity work.
Request this serviceThe Gateway advantage
Routine turnaround of 24–48 hours for standard templates and primers.
PCR products, plasmids, purified gDNA fragments and cloned inserts.
Standard primers stocked for common vectors and barcodes; custom primers accepted.
Chromatogram (.ab1) files delivered with quality scores alongside edited consensus text.
Capillary electrophoresis on Applied Biosystems SeqStudio-class instruments.
Plate-scale submissions for colony screens, library verification and biodiversity surveys.
Laboratory workflow & QA
Step 1
Register templates and primers — guidance on concentrations provided at checkout.
Step 2
Concentration and purity checked; failures flagged before chemistry is consumed.
Step 3
BigDye-style cycle sequencing reactions run with your or standard primers.
Step 4
Fragments resolved on capillary electrophoresis for base-called traces.
Step 5
Chromatograms and consensus text delivered to your project space, typically within 48h.
Beyond the FASTQ
Every read ships with the raw trace and an edited consensus — ready for alignment against your reference.
Deliverables include
Chromatogram (.ab1) files
Edited consensus (FASTA/text)
Quality scores
Chromatogram (.ab1) files
Edited consensus (FASTA/text)
Quality scores
Primer-site annotation
Alignment-ready outputs
Plate manifests for batch runs
Primer-site annotation
Alignment-ready outputs
Plate manifests for batch runs
Figures & files delivered with every project — hover to pause
Applications
Confirm engineered plasmids before downstream experiments.
Validate NGS variant calls and edited alleles at single loci.
COI/16S/ITS barcodes for taxonomy, forensics and food authenticity.
Targeted reads for markers, repeats and known loci.
FAQs
Explore related sequencing solutions
Tell us about your templates — routine reads return within 48 hours with traces and edited consensus.
Share your samples and goals — a doctoral-level scientist responds within one business day with a study plan and competing blind quotes.
Administered by Syntax of Life Private Limited
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